Effect of low-dose cyclophosphamide on endoplasmic reticulum stress and inflammatory reaction of acute renal ischemia reperfusion injury

Y. Long, P. Wang, H. Sun, H. Wang, P. Chen, JS. Zhao

Department of Anesthesia, Nanjing Jingdu Hospital, No. 34 Biao, Yanggongjing, Nanjing, Jiangsu Province, China. zhaojs61@163.com

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• Pharmacology

OBJECTIVE: To explore the effect of low-dose cyclophosphamide (CP) on endoplasmic reticulum stress (ERS) and inflammatory reaction of acute renal ischemia reperfusion injury (IRI), providing new ideas for clinical treatment.

MATERIALS AND METHODS: Sixty healthy adult male mice were selected and divided randomly into sham operation group (n = 20), IRI group (n = 20 cases), and the experimental group (n = 20 cases). Mice in the experimental group were pretreated with low-dose CP and acute IRI model construction. Intercellular adhesion molecule-1 (ICAM-1) immunohistochemical staining was conducted after the 24h and the detection of CHOP protein by Western blot method. Postoperative 3h and 7 day survival rate, the results of ICAM-1 immunohistochemistry staining and the gray values of C/EBP homologous protein (CHOP) of mice were compared between the three groups.

RESULTS: The survival rates of mice in the IRI group and the experimental group after operation were decreased significantly (p < 0.05). However, the survival rates of mice in the experimental group were higher than that in the IRI group. The differences were significant (p < 0.05). The expression of ICAM-1 was significantly reduced (p < 0.05). The difference was statistically significant. The expression of ICAM-1 was significantly reduced (p < 0.05). The gray values of mice in the IRI group and the experimental group were significantly increased. The difference was statistically significant (p < 0.05). And the gray value in the IRI group increased more than that of the experimental group. The difference was statistically significant (p < 0.05).

CONCLUSIONS: The pretreatment with low-dose CP for mice with acute renal IRI can reduce effectively the ERS and inflammation, which reduces kidney damage for mice and have good effect on prolonging its life. It provides a good way for the guidance of clinical research.

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