Relationship between CD4+CD25+ Treg and expression of HIF-1α and Ki-67 in NSCLC patients

L.-Y. He, L. Li, M.-L. Guo, Y. Zhang, H.-Z. Zhang

People’s Hospital of Hebei Province, Shijiazhuang, Hebei Province, China. hongzhenzhang456@sohu.com

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• Oncology

OBJECTIVE: To investigate the relationship between the CD4+CD25+ Treg cell proportion in the peripheral blood and the clinicopathologic features of non-small cell lung cancer (NSCLC) patients and hypoxia-inducible factor-1α (HIF-1α), Ki-67 expression.

PATIENTS AND METHODS: Flow cytometry was used to measure the CD4+CD25+ Treg cell level in peripheral blood and immunohistochemical staining used to detect the expression of HIF-1α and Ki-67 protein in cancer tissue of each of 50 NSCLC patients.

RESULTS: The level of CD4+CD25+ Treg cell in peripheral blood was related to pathologic grades (t = 3.265, p = 0.006) and clinical stage (t = 4.417, p = 0. 001) of NSCLC instead of to patient’s gender and pathologic type of tumor (p > 0.05). The level of CD4+CD25+ Treg cell was positively correlated with the expression of HIF-1α (r = 0.711, p = 0.003) and Ki-67 (r = 0.517, p = 0.04), respectively.

CONCLUSIONS: CD4+CD25+ Treg cell can be used as a predictor of immune status and prognosis of NSCLC patients and the levels of HIF-1α and Ki-67 protein expression may relate to inhibition of immune cells.

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