Effect of Fe2+ and Fe3+ ions on human plasma cholinesterase activity

M. Karami 1, M.A. Ebrahimzadeh 1, M.R. Mahdavi 2, A. Kazemi 1

1 Pharmaceutical Sciences Research Center, School of Pharmacy and Traditional and Complementary Medicine Research Center; 2 Faculty of Paramedical, Mazandaran University of Medical Sciences, Sari (Iran)

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• Pharmacology

Abstract. – Objectives: Metals such as iron are needed for physiological functions of the body. However, their excess may cause serious damage including poisoning. The effects of Fe2+ and Fe3+ ions on human plasma cholinesterase activity were investigated.
Material and Methods: Plasma were obtained (n=24) from healthy male volunteers, and treated with five various concentrations of Fe2+ and Fe3+ ions. Activity of butyryl cholinesterase (BChE) was assayed according to colorimetric Ellman procedure. The activity was calculated as micromole of hydrolyzed substrate, per minute in each ml.
Results: Both ferric (Fe3+) and ferrous (Fe2+) ions significantly decreased the BChE activity in all tested concentrations. The effect of ferric (Fe3+) ions on the reduction of plasma enzyme activity was more pronounced than ferrous (Fe2+) ions (p Conclusions: The present paper represents the effect of Fe2+ and Fe3+ ions on ButyrylChE activity in human plasma. It reveals that both ions suppress BChE activity.

Corresponding Author: Mohamamd Ali Ebrahimzadeh, Ph.D; e-mail: zadeh20@yahoo.com

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