Effects of polyunsaturated fatty acids on endometrial carcinoma Ishikawa cells

X.-Y. Lu, T. Zeng

Department of Obstetrics and Gynecology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, China.  xy_lu68@aliyun.com

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• Oncology

OBJECTIVE: This study aimed to explore the effects and mechanisms of polyunsaturated fatty acids in the endometrial carcinoma Ishikawa cells.

MATERIALS AND METHODS: The Ishikawa cells were treated with n-6 PUFAs, n-3 PUFAs, or the mixture of n-6/n-3 PUFAs at a ratio of 1:1/10:1. Cell proliferation was quantified by MTT assay, while expressions of Akt, mTOR and VEGF mRNAs by PCR.

RESULTS: Treatment with n-6 PUFAs and 10:1 n-6/n-3PUFAs markedly promoted cell proliferation and up-regulated expressions of Akt, mTOR and VEGF mRNAs. Further, n-3 PUFAs and 1:1 n-6/n-3 PUFAs markedly suppressed cell proliferation and down-regulated expressions of Akt, mTOR and VEGF mRNAs.

CONCLUSIONS: Different ratios of n-6/n-3PUFAs exhibit differential effects on the endometrial carcinoma Ishikawa cells through the PI3k/Akt/mTOR signaling pathway and differentially regulate VEGF expression.

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