The influence of angiotensin-converting enzyme 2 gene polymorphisms on type 2 diabetes mellitus and coronary heart disease

J. Chaoxin, S. Daili, H. Yanxin, G. Ruwei, W. Chenlong, T. Yaobin

Department of Clinical Laboratory, the Third People’s Hospital, Nanhai District, Foshan City, Guangdong, China. jiangchaoxinchx@hotmail.com

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• Cardiology

BACKGROUND: Diabetes mellitus is a chronic metabolic disorder that results from various genetic and environmental factors, and type 2 diabetes mellitus (T2DM) combined with coronary heart disease (CHD) is one of the most common chronic complications in diabetes.

AIM: To explore the correlation between the polymorphism of angiotensin-converting enzyme 2 (ACE2) and T2DM combined with CHD.

PATIENTS AND METHODS: A total of 120 patients with T2DM and 93 patients with T2DM and CHD were selected to participate in this study. And polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) was used to analyze the polymorphism of G8790A in ACE2 gene. Meanwhile, the clinical, biochemical and echocardiographic data were also analyzed.

RESULTS: There was no significant difference between T2DM group and T2DM combined with CHD group in genotype and allele frequencies (p > 0.05). And for different genotypes, both groups had no significant difference in the age, BMI, blood lipid, fasting blood glucose, EF ratio, E/A ration, LVPWTd and LVEDd (p > 0.05). But for male patients in T2DM combined with CHD group, the IVSTd, LVPWTd and urine protein level of allele G were significantly higher than that of allele A with p values less than 0.01, 0.05 and 0.05, respectively.

CONCLUSIONS: Genetic polymorphism of G8790A in ACE2 gene plays an important role on the pathogenesis of male patients with T2DM and CHD by changing the levels of IVSTd, LVPWTd and urine protein. Therefore, our results reveal the role of ACE2 polymorphism in the pathogenesis of T2DM with CHD and identify several potential biomarkers for this disease.

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