Soft tissue regeneration in animal models using grafts from adipose mesenchymal stem cells and peripheral blood fibrin gel

T.D. Huynh, H.K. Nguyen, A.M. Inchingolo, H.L. Bao Tran, G. Dipalma, A. Mancini, K. Cao Diem Nguyen, M.G. Balzanelli, P. Distratis, R. Lazzaro, T.A. Nguyen Ho, E.M. Serlenga, V. Hung Pham, G. Romanos, B.T. Nguyen, B. Rapone, F. Inchingolo, C. Isacco Gargiulo, T. Cong Tran

Department of Histology, Embryology, and Genetics, Pham Ngoc Thach University of Medicine, Ho Chi Minh City, Vietnam. francesco.inchingolo@uniba.it

---

• Stem Cells

OBJECTIVE: Our study aimed to evaluate the effect of soft tissue regeneration in nude mice using grafts made from the combination of adipocytes from fat tissue mesenchymal stem cells and fibrin gel from peripheral blood.

MATERIALS AND METHODS: Mesenchymal stem cells were isolated from adipose tissue and identified according to ISCT criteria. The scaffold used was fibrin obtained from peripheral blood. The grafts in this study were generated by transferring mesenchymal stem cells onto a fibrin scaffold. Two types of grafts, the research sample (fibrin scaffold containing adipocytes differentiated from mesenchymal stem cells) and the control sample (fibrin scaffold only), were grafted under the dorsal skin of the same mouse. After each research period, samples were collected and evaluated by histological methods to observe the existence and growth of cells inside the grafts.

RESULTS: The results showed that the study group’s graft integrated better within the tissue when compared with the control group. In addition, the grafts in the study group showed the presence of cells with characteristic morphology of adipocytes one week after transplantation. In contrast, control samples showed dimorphous shapes and features mainly composed of non-homogenous fragments.

CONCLUSIONS: These initial conclusions might be considered a first step in generating safe bio-compatible engineered grafts specifically usable in post-traumatic tissue regeneration procedures.

Free PDF Download