A long non-coding RNA, ELFN1−AS1, sponges miR-1250 to upregulate MTA1 to promote cell proliferation, migration and invasion, and induce apoptosis in colorectal cancer

L.-Q. Zhai, X.-X. Wang, C.-X. Qu, L.-Z. Yang, C.-M. Jia, X.-C. Shi

Department of Pathology, Shanxi Provincial People’s Hospital, Taiyuan, Shanxi Province, China. LiqinZhai8080@163.com

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• Oncology

OBJECTIVE: Long non-coding RNA (lncRNA), is essential for the development and progression of cancers. LncRNA regulates target gene expression by sponging the corresponding microRNA (miRNA) during tumorigenesis. This work aimed to explore the role of one lncRNA, ELFN1−AS1, in colorectal cancer (CRC) development and elucidate the pertinent signaling pathway.

PATIENTS AND METHODS: First, we found that ELFN1−AS1 was highly abundant in the human CRC tissues and cell lines. Silence of ELFN1−AS1 expression reduced cell proliferation, colony formation, migration and invasion, while inducing apoptosis in vitro; moreover, knockdown of ELFN1−AS1 decreased the size and weight of tumor in vivo.

RESULTS: Luciferase reporter assay revealed that ELFN1−AS1 interacted with miR-1205 and suppressed its expression. In addition, miR-1205 could bind to the 3ʹ untranslated region (3ʹ-UTR) of Metastasis Associated Protein1 (MTA1) and inhibited ELFN1−AS1 expression. More importantly, overexpression of MTA1 completely rescued the phenotype of ELFN1−AS1 knockdown.

CONCLUSIONS: In sum, our study demonstrated that ELFN1−AS1 sponges miR-1205 to upregulate MTA1, which is essential for CRC cell proliferation, migration, and invasion as well as apoptosis induction.

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