LINC00641 regulates prostate cancer cell growth and apoptosis via the miR-365a-3p/VGLL4 axis

W.-H. Liu, J.-J. Lu, R.-K. Yu, L. Zhou, Q. Yu, D.-F. Li, Q.-H. Zhu

Department of Urology, Beilun District People’s Hospital, Ningbo, China. qianhao_zhu@126.com

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• Oncology

OBJECTIVE: Long non-coding RNA (lncRNA) was frequently abnormally expressed in cancers. LINC00641 was reported to play crucial roles in regulating tumor progression. However, its role in prostate cancer (PCa) has not been fully explored.

PATIENTS AND METHODS: In this work, proliferation, invasion and apoptosis assays were performed to detect the biological roles of LINC00641 in PCa. Bioinformatic analyses, Luciferase activity reporter assay, and rescue experiments were performed to investigate the potential mechanisms of LINC00641 in PCa. Expression levels of LINC00641, microRNA-365a-3p (miR-365a-3p), and vestigial like family member 4 (VGLL4) in PCa tissues and normal tissues were analyzed at ENCORI.

RESULTS: We found LINC00641 and VGLL4 was reduced, while miR-365a-3p was elevated expression in PCa tissues compared with normal tissues. LINC00641 overexpression inhibited growth and invasion abilities of PCa cells in vitro. Functional assays revealed that miR-365a-3p/VGLL4 pair was the downstream targets of LINC00641.

CONCLUSIONS: The findings of our work provided evidence that LINC00641 serves as a tumor suppressive lncRNA in PCa by regulating miR-365a-3p/VGLL4 axis.

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