MicroRNA-219 inhibits cell viability and metastasis in papillary thyroid carcinoma by targeting EYA2

H.-M. Liu, A.-B. Dong, H. Hua, Y.-H. Sun, J.-R. Wang, Q.-Q. Yu, J.-H. Zhang, W.-H. Sun

Department of Oncology, The Affiliated Hospital of Qingdao University, Qingdao, China. weixianlu335605@163.com

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• Oncology

OBJECTIVE: In recent years, the incidence of papillary thyroid carcinoma (PTC) has increased. Many microRNAs (miRNAs) have been found to regulate PTC progression. However, the regulatory mechanism of miR-219 remains unclear in PTC. Therefore, the purpose of this study is to explore the function of miR-219 in PTC.

PATIENTS AND METHODS: Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and Western blot analysis were used to detect the expression of miR-219 and eyes absent homologue 2 (EYA2). The function of miR-219 was investigated by methyl thiazolyl tetrazolium (MTT) and transwell assays. The relationship between miR-219 and EYA2 was confirmed by Dual-Luciferase reporter assay.

RESULTS: MiR-219 expression was reduced and was associated with TNM stage and lymph node metastases in PTC patients. Functionally, overexpression of miR-219 restrained the viability and metastasis of PTC cells. In addition, miR-219 induced apoptosis and blocked EMT in PTC cells. Furthermore, miR-219 was confirmed to directly target EYA2 and inhibited its expression in PTC. More importantly, the upregulation of EYA2 impaired the inhibitory effect of miR-219 in PTC.

CONCLUSIONS: MiR-219 inhibits the viability and metastasis of PTC cells by downregulating EYA2.

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