Effect of hsa_circ_RNA0023397 regulation of miR-106b expression on proliferation and apoptosis of esophageal cancer cells

X.-Y. Li, B. Yang, Y. Sun, Y. Gao, L. Lu

Department of Gastroenterology, Taizhou People’s Hospital, Taizhou, China. d111030@163.com

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• Oncology

OBJECTIVE: To explore the potential function of a candidate circular ribonucleic acid (circRNA) [human serum albumin (hsa)_circ_RNA0023397] in esophageal cancer cells.

MATERIALS AND METHODS: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to detect the expression level of hsa_circ_RNA0023397 in three esophageal cancer cell lines (KYSE-150, ECA109, and TE-1), which was compared with that in normal human esophageal epithelial cell line (HET-1A). The expression plasmid of hsa_circ_RNA0023397 was constructed, and the effect of overexpression of hsa_circ_RNA0023397 on cell proliferation was determined by cell counting kit-8 (CCK-8) and colony formation assay. The effect of overexpression of hsa_circ_RNA0023397 on cell apoptosis was detected by flow cytometry. Further bioinformatics analysis and Luciferase reporter gene analysis were carried out to explore the role of hsa_circ_RNA0023397 as a sponge of micro RNAs (miRNAs).

RESULTS: Compared with that in normal human esophageal epithelial cell line HET-1A, the expression of hsa_circ_RNA0023397 was down-regulated in three esophageal cancer cell lines in vitro. Overexpression of hsa_circ_RNA0023397 overtly inhibited KYSE-150 cell proliferation and promoted its apoptosis. Bioinformatics prediction and Luciferase reporter gene assay confirmed that hsa_circ_RNA0023397 could bind to miR-160b. MiR-106b participated in hsa_circ_RNA0023397-mediated inhibition of proliferation of esophageal cancer KYSE-150 cells.

CONCLUSIONS: Hsa_circ_RNA0023397 is down-regulated in esophageal cancer cells and can act as miR-106b to affect the biological function of esophageal cancer cells.

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