Effects of IFNG-AS1 and ANRIL on intestinal epithelial cells and their relationship with colitis

Q. Huang, C.-M. Shi, Y.-L. Miao, Y. Chen

Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Yunnan Institute of Digestive Disease, Kunming, P.R. China. cygznf@sina.com

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• Gastroenterology

OBJECTIVE: To explore the effects of IFNG-AS1 and ANRIL on intestinal epithelial cells and their relationship with colitis.

PATIENTS AND METHODS: From May 2017 to May 2019, 118 colitis patients admitted to our hospital were selected as the research group (RG), and 124 healthy controls were selected as the control group (CG). In addition, the normal intestinal epithelial cells HIEC and HIEC-6 were purchased to detect the IFNG-AS1 and ANRIL in the peripheral blood of patients in the two groups, and the effects of IFNG-AS1 and ANRIL on the intestinal epithelial cells were analyzed.

RESULTS: IFNG-AS1 and ANRIL were highly expressed in colitis (p<0.050), and their combined detection had good diagnostic value for the occurrence of colitis and complications (p<0.050). In intestinal epithelial cells transfected with IFNG-AS1 and ANRIL, it was found that inhibition of IFNG-AS1 and ANRIL remarkably increased the proliferation and decreased the apoptosis of intestinal epithelial cells (p<0.050).

CONCLUSIONS: IFNG-AS1 and ANRIL are highly expressed in colitis, and inhibiting their expression can promote the proliferation of intestinal epithelial cells and reduce apoptosis, which may be potential therapeutic targets for Crohn’s colitis in the future.

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