CircRNA_010763 promotes growth and invasion of lung cancer through serving as a molecular sponge of miR-715 to induce c-Myc expression

P. Zhang, X.-F. Xue, X.-Y. Ling, Q. Yang, Y. Yu, J. Xiao, Z.-N. Wang

Department of Cardiothoracic Surgery, Changzheng Hospital, Navy Medical University, Shanghai, China.  wangzn007@smmu.edu.cn

---

• Oncology

OBJECTIVE: This study aims to investigate the regulatory effect of circRNA_010763 on the growth and invasion of non-small cell lung cancer (NSCLC).

PATIENTS AND METHODS: qRT-PCR was performed to detect the expressions of circRNA_010763 and c-Myc in human NSCLC tissues and cells. CCK-8 assay was performed to evaluate the A549 cells proliferation and transwell assay was performed to evaluate the A549 cells migration. The correlation between miR-715 and circRNA_010763 was detected by statistical analysis. Bioinformatics prediction and Luciferase assay were performed to explore the interaction and binding site of circRNA_010763 and miR-715, miR-715 and c-Myc, respectively.

RESULTS: We found that both circRNA_010763 and c-Myc were upregulated in human NSCLC tissues and cells. qRT-PCR and CCK-8 assay showed that circRNA_010763 expression is associated with the proliferation of NSCLC cells. Transwell assay showed that circRNA_010763 regulated the migration ability of NSCLC cells. The bioinformatics prediction and Luciferase assay demonstrated that circRNA_010763 can sponge with miR-715, serving as a molecular sponge to further regulate the expression of c-Myc.

CONCLUSIONS: In this study, we found that circRNA_010763 was highly expressed in human NSCLC tissues, which could promote tumor proliferation, migration and invasion through serving as a molecular sponge by modulating the inhibitory effect of miR-715 on oncogene c-Myc.

Free PDF Download