MicroRNA-381 inhibits metastasis and epithelial-mesenchymal transition of glioblastoma cells through targeting LEF1

R.-Q. Min, Q. Ma

Physical Examination Rehabilitation Center, Civil Aviation General Hospital, Beijing, China. xiaoqian0306@sina.com

---

• Oncology

OBJECTIVE: Glioblastoma is a common intracranial malignancy that is extremely harmful to human life and health. Various microRNAs (miRNAs) have been reported to be involved in the progression of glioblastoma, except miR-381. Therefore, the purpose of this study is to investigate the role of miR-381 in glioblastoma.

MATERIALS AND METHODS: The expression of miR-381 and LEF1 (lymphoid enhancer-binding factor-1) was quantified using quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and Western blot analysis. Transwell and Dual-Luciferase reporter assays were used to investigate the regulatory mechanism of miR-381/LEF1 in glioblastoma.

RESULTS: Downregulation of miR-381 was observed in A172 cells. In addition, the overexpression of miR-381 restrained migration and invasion of glioblastoma cells. Furthermore, overexpression of miR-381 inhibited epithelial-mesenchymal transition (EMT) in A172 cells. Further, miR-381 was confirmed to directly target LEF1 and negatively regulates its expression in glioblastoma cells. Downregulation of LEF1 also inhibited cell migration, invasion, and EMT in glioblastoma cells. More importantly, the upregulation of LEF1 abolished the inhibitory effect of miR-381 in glioblastoma cells.

CONCLUSIONS: MiR-381 inhibits cell metastasis and EMT in glioblastoma by suppressing LEF1 expression.

Free PDF Download