Circ-100338 induces angiogenesis after myocardial ischemia-reperfusion injury by sponging miR-200a-3p
H. Chang, Z.-B. Li, J.-Y. Wu, L. Zhang Department of Cardiovascular Medicine, China-Japan Union Hospital, Jilin University, Changchun, Jilin, China. Zhanglei99@jlu.edu.cn
OBJECTIVE: To investigate the effect of circular RNA circ-100338 on angiogenesis of human umbilical vein endothelial cells (HUVEC) cells after hypoxia/reoxygenation (H/R) and its molecular mechanism.
MATERIALS AND METHODS: We evaluated the role of circ-100338 in coronary artery endothelial cells using human coronary endothelial cells (HCAEC). Then, we verified the function of circ-100338 in HUVEC cells through cell counting kit-8 (CCK8), scratch test, Tube forming experiment, 5-Ethynyl-2’-deoxyuridine (EdU) staining. Dual-Luciferase reporter gene experiment and RNA Pull-Down experiments were used to detect the binding effect of circ-100338 and miR-200a-3p, miR-200a-3p and FUS.
RESULTS: QRT-PCR results showed that the expression of circ-100338 decreased in HCAEC after H/R treatment. Overexpression of circ-100338 promotes angiogenesis. The Dual-Luciferase reporter gene assay and RNA pull-down assay consistently indicated the specific binding effect between circ-100338 and miR-200a-3p, miR-200a-3p and FUS, and circ-100338 promoted the angiogenesis phenotype in HUVEC cells.
CONCLUSIONS: CircRNA-100338 may inhibit the function of miRNA-200a-3p by combining with miRNA-200a-3p, and then miRNA-200a-3p plays a role in regulating FUS, thereby regulating the state of angiogenesis.
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To cite this article
H. Chang, Z.-B. Li, J.-Y. Wu, L. Zhang
Circ-100338 induces angiogenesis after myocardial ischemia-reperfusion injury by sponging miR-200a-3p
Eur Rev Med Pharmacol Sci
Year: 2020
Vol. 24 - N. 11
Pages: 6323-6332
DOI: 10.26355/eurrev_202006_21530