Circ_001680 stimulates glioma progression with the involvement of miR-186-5p

J. Li, D. Liu, Z. Cui

Department of Neurosurgery, Shanghai Fifth People’s Hospital, Fudan University, Shanghai, China. cuizheng007@163.com

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• Oncology

OBJECTIVE: This study aims to explore the influences of circ_001680 on glioma proliferation and metastasis, and the underlying mechanism.

PATIENTS AND METHODS: Circ_001680 levels in 40 pairs of glioma and normal tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between circ_001680 level and clinical indicators in glioma patients was analyzed. After knockdown of circ_001680 in U251 and U87 cells by transfection of sh-circ_001680, changes in proliferative, migratory and invasive abilities were assessed by cell counting kit-8 (CCK-8), transwell and wound healing assay, respectively. The binding relationship between circ_001680 and miR-186-5p was predicted by online bioinformatics tools and detected by Dual-Luciferase reporter assay. In addition, their synergistic regulations on glioma progression were explored by rescue experiments.

RESULTS: Circ_001680 was highly expressed in glioma tissues than normal ones. Incidences of lymphatic metastasis and distant metastasis were higher in glioma patients expressing higher level of circ_001680 than those with a lower level. Cell function experiments uncovered that knockdown of circ_001680 inhibited proliferative and metastatic abilities in U251 and U87 cells. MiR-186-5p was downregulated in glioma tissues and negatively correlated to that of circ_001680. Knockdown of miR-186-5p could abolish the inhibitory effects of silenced circ_001680 on glioma progression.

CONCLUSIONS: Circ_001680 stimulates proliferative and metastatic abilities in glioma by negatively regulating miR-186-5p level. High level of circ_001680 is closely linked to lymphatic metastasis, distant metastasis and poor prognosis in glioma patients.

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