SLC5A8 regulates the biological behaviors of cervical cancer cells through mediating the Wnt signaling pathway

X.-M. Zhang, Q.-H. Meng, F.-F. Kong, K. Wang, L.-J. Du

Department of Obstetrics, Jinan Second Maternal and Child Health Hospital, Jinan, China. 5116816@qq.com

 

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• Oncology

OBJECTIVE: This study aims to investigate the role of solute transport family 5 member 8 (SLC5A8) in the progress of cervical cancer (CC) to provide a theoretical basis for the treatment of CC.

PATIENTS AND METHODS: Tissues were obtained from 58 patients diagnosed with CC in our hospital. Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) analysis was used to detect the expression level of SLC5A8 in CC tissues and cell lines. SLC5A8 level was up-regulated by transfection of SLC5A8 overexpression plasmid. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay and flow analysis were designed to measure the cell proliferation, cell cycle, and apoptosis of CC cells.

RESULTS: The mRNA expression of SLC5A8 was down-regulated in CC tissues and cell lines. Transfection of SLC5A8 overexpression plasmid successfully over-expressed SLC5A8. In addition, an inhibited activation of Wnt signaling pathway was detected in CC cells after over-expression of SLC5A8. Besides, decreased proliferation activity and increased apoptosis were also observed in CC cells overexpressing SLC5A8 plasmid. Moreover, the impaired proliferation activity and increased apoptosis proportion of CC cells induced by SLC5A8 over-expression could be counteracted by the Wnt signaling pathway activator LiCl.

CONCLUSIONS: SLC5A8 alleviates the progression of CC by regulating the Wnt signaling pathway.

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