Caprin1 targeted by circular circ_0000885 in the tumor progression of osteosarcoma

X.-Y. Liu, M. Li, B. Dong, Q. Feng

Department of Orthopedics, Cangzhou People’s Hospital, Cangzhou, China. fengqing1974@126.com

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• Oncology

OBJECTIVE: Recent studies have revealed that circular RNAs (circRNAs) participate in the progression and development of many human diseases. Therefore, the purpose of this study was to uncover the role of circ_0000885 in the development of osteosarcoma and to explore the possible underlying mechanism.

PATIENTS AND METHODS: Circ_0000885 expression in osteosarcoma tissues and cells was detected by Real Time-quantitative Polymerase Chain Reaction (RT-qPCR). The knockdown of circ_0000885 was conducted in osteosarcoma cells. Subsequently, its function in the progression of osteosarcoma was determined by cell proliferation assay, colony formation assay, and cell cycle assay, respectively. Furthermore, the underlying mechanism was explored by RT-qPCR and Western blot.

RESULTS: Circ_0000885 was highly expressed in osteosarcoma tissues than that of adjacent tissues. Cell growth was significantly suppressed, and the distribution of cell cycle was regulated after circ_0000885 knock-down in vitro. Besides, both the mRNA and protein expressions of Caprin1 were significantly downregulated via silence of circ_0000885. Furthermore, Caprin1 was positively correlated with circ_0000885 in osteosarcoma tissues.

CONCLUSIONS: Circ_0000885 could enhance osteosarcoma cell proliferation and regulate cell cycle by upregulating Caprin1, which might contribute to the therapy for osteosarcoma.

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