LncRNA UCA1 regulates proliferation, migration and invasion of cervical cancer cells by targeting miR-145

H. Wei, Y.-Q. Qiu, Q.-S. Zeng, S.-F. Wang, C.-J. Yi

Department of Obstetrics and Gynecology, The First Clinical Medicine College of Yangtze University, Jingzhou, Hubei, China. youyoucheng15@163.com

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• Oncology

OBJECTIVE: Finding a key target for the development of cervical cancer is conducive to the treatment of cervical cancer. LncRNA UCA1 plays a role in multiple tumors, such as the digestive tract and bladder. However, whether LncRNA UCA1 plays a role in cervical cancer is unclear.
PATIENTS AND METHODS: The tumor tissues (tumor group) and adjacent tissues (normal group) of cervical cancer patients were collected for analysis of expression of LncRNA UCA1 and miR-145 by Real Time-Polymerase Chain Reaction (RT-PCR). Cervical cancer HeLa cell line was cultured and divided into NC group, si-UCA1 group, and si-UCA1+ miR-145 inhibitor group. It was followed by the analysis of LucRNA UCA1 expression by Real Time-PCR, cell proliferation by MTT assay, cell migration and invasion by respective cell scratch, transwell chamber assay, and targeted relationship between UCA1 and miR-145 by Dual-Luciferase activity.
RESULTS: LncRNA UCA1 expression was significantly increased in cervical cancer and miR-145 expression was decreased compared with the normal group (p<0.05). There was a negative correlation between them (p<0.05). The downregulation of UCA1 significantly inhibited tumor cell proliferation, migration, and invasion compared with NC group (p<0.05). MiR-145 is the target miRNA of UCA1. The addition of miR-145 inhibitor reversed the effect of UCA1 siRNA on downregulating UCA1 expression, and the difference was statistically significant compared with the si-UCA1 group (p<0.05).
CONCLUSIONS: LncRNA UCA1 expression is significantly increased in cervical cancer and miR-145 expression is decreased with a negative correlation between them. Lnc-RNA UCA1 regulates cervical cancer cell proliferation, migration, and invasion by targeting miR-145.

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