Circular RNA circ_0103552 promotes the invasion and migration of thyroid carcinoma cells by sponging miR-127

F.-B. Zheng, D. Chen, Y.-Y. Ding, S.-R. Wang, D.-D. Shi, Z.-P. Zhu

Department of Nuclear Medicine, Qingdao Municipal Hospital, Qingdao, China. zhuzongping1024@126.com

---

• Oncology

OBJECTIVE: The aim of this study was to examine the regulatory role of circ-0103552 in the procession of thyroid carcinoma (TC) and the related underlying mechanisms.

PATIENTS AND METHODS: The tissues were obtained from 56 patients diagnosed with TC in our hospital. Nthy-ori3-1 cell line and TC cell lines (TPC-1, SW579, 8505C) were purchased from American Type Culture Collection (ATCC). Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was conducted to analyze the expression of circ-0103552 in TC tissues and cell lines. Inhibition of circ-0103552 was achieved by circ-0103552 siRNA. Dual-Luciferase report assay was performed to confirm the potential target miRNA of circ-0103552. The transwell assay and wound-healing assay were designed to examine the invasion and migration ability of TC cells, respectively.

RESULTS: Circ-0103552 was detected to be upregulated in TC tissues, as well as in TC cell lines, including TPC-1, SW579, and 8505C. The knockdown of circ-0103552 significantly reduced the invasion and migration ability in TC cells. It was predicted using the circular RNA database that microRNA-127 (miR-127) was a target miRNA of circ-0103552, which was confirmed by the Dual-Luciferase assay. Further studies revealed that circ-0103552 was involved in the invasion and migration of TC by sponging miR-127.

CONCLUSIONS: The present study demonstrated that circ-0103552 acts as a regulator in the invasion and migration of TC by sponging miR-127.

 

Free PDF Download