MiR-135b-5p inhibits the progression of malignant melanoma cells by targeting RBX1

X.-H. Zhang, Z.-M. Xin

Department of Burn and Plastic Surgery, Affiliated Success Hospital of Xiamen University (The 174th Hospital of PLA), Xiamen, China. zxyyxzm@163.com

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• Oncology

OBJECTIVE: The aim of this study was to investigate the potential effects of microRNA-135b-5p (miR-135b) on the development of malignant melanoma (MM) and the relevant mechanism.

PATIENTS AND METHODS: The expression level of miR-135b in MM tissues and cells was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Online prediction software and luciferase reporter assays were used to predict and verify the possible target of miR-135b, respectively. Furthermore, the effects of the miR-135b on MM A375 cells were determined by Western blotting, MTT, and transwell assays.

RESULTS: MiR-135b was significantly down-regulated in MM. RING-box protein 1 (RBX1) was verified as a direct target of miR-135b. Subsequent experiments showed that down-regulation of RBX1 resulted from miR-135b up-regulation could significantly inhibit the proliferation, invasion, and migration abilities of MM cells.

CONCLUSIONS: MiR-135b inhibited the progression of MM by targeting RBX1. Our findings revealed that miR-135b/RBX1 might be a potential therapeutic target for the treatment of MM.

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