Effect of GREM 1 gene on chemoradiotherapy sensitivity of cervical squamous carcinoma cells

H. Miao, W.-B. Song, H. Zhu, Q. Wang, Y. Tian

The Second Affiliated Hospital of Soochow University, Suzhou, P. R. China. dryetian@126.com

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• Oncology

OBJECTIVE: To investigate the effect of GREM1 on the sensitivity of cervical squamous carcinoma cells to radiotherapy and chemotherapy.

PATIENTS AND METHODS: The resected cancer tissues and paracancerous tissues of patients with cervical carcinoma were collected. The expressions of GREM1 protein and mRNA in SiHa cell line of cervical squamous carcinoma were tested by Western blot and reverse transcription-polymerase chain reaction (qRT-PCR). The effect of GREM1 on chemotherapy sensitivity of SiHa cells was tested by MTT assay. SiHa cells were irradiated with different doses of X-ray, and the changing trend of GREM1 in cell lines was observed.

RESULTS: Compared with paracancerous tissues, the expression level of GREM1 in cervical squamous carcinoma was significantly higher than that in paracancerous tissues (p<0.05). After adding different concentrations of chemotherapeutic drugs, the relative survival rate of SiHa cells overexpressing GREM1 group was significantly increased. After high-energy X-ray irradiation with different radiation doses of SiHa cells, the expression levels of GREM1mRNA and protein in SiHa cell lines showed a significant downward trend. GREM1 was highly expressed in cervical squamous carcinoma.

CONCLUSIONS: Down-regulating GREM1 expression can increase the chemotherapy sensitivity of SiHa cells. GREM1 may be related to the radiotherapy sensitivity of cervical squamous carcinoma.

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