LINC00467 promotes proliferation and invasion in glioma via interacting with miRNA-485-5p

X.-H. Jiang, Y.-Y. Liu

Department of Neurosurgery, Sichuan Academy of Medical Sciences, Sichuan Provincial People’s Hospital, Chengdu, Sichuan Province, China. 1450064113@qq.com

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• Oncology

OBJECTIVE: Long non-coding RNA (lncRNA) LINC00467 was found to be upregulated in glioma tissues by analyzing The Cancer Genome Atlas (TCGA) database. This study aims to uncover the biological role of LINC00467 in influencing the progression of glioma and to provide novel directions for clinical treatment of glioma.
PATIENTS AND METHODS: The expression levels of LINC00467 in glioma tissues were analyzed in the downloaded Gene Expression Profiling Interactive Analysis (GEPIA) dataset. Meanwhile, LINC00467 levels in glioma tissues collected in our hospital and glioma cell lines were determined as well. Proliferative, apoptotic, and invasive changes in U87 and U251 cells transfected with si-LINC00457 or si-NC were assessed. The binding between LINC00467 and microRNA-385-5p (miR-385-5p) was predicted through online bioinformatics and verified by the Dual-Luciferase reporter gene assay. The interaction between LINC00467 and miR-385-5p involved in the progression of glioma was finally verified through rescue experiments.
RESULTS: LINC00467 was upregulated in glioma. The knockdown of LINC00467 attenuated proliferative and invasive abilities, and induced apoptosis in U87 and U251 cells. LINC00467 could bind miRNA-485-5p and negatively regulate its level. Moreover, miRNA-485-5p was responsible for the development of glioma influenced by LINC00467.
CONCLUSIONS: LINC00467 aggravates the progression of glioma by negatively regulating miRNA-485-5p, which may be a potential therapeutic target for glioma.

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