Effect of miR-200c on migration and proliferation of breast cancer MDA-MB-231 cells and BT-549 cells and the possible mechanism

Y. Lei, Y. Ma, Y. Liu, X.-F. Wang

Department of Medical Oncology, Shaanxi Provincial People’s Hospital, Xi’an, P.R. China. rzkqa7@163.com

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• Oncology

OBJECTIVE: This study was designed to investigate the effects of miR-200c on the migration and proliferation of breast cancer MDA-MB-231 cells and BT-549 cells and the possible mechanisms.
MATERIALS AND METHODS: The effects of miR-200c on the proliferation and migration of highly invasive human breast cancer MDA-MB-231 and BT-549 cells were investigated by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) MTT and wound-healing assay. Effects of miR-200c on the expression of adhesion molecules in cells of 2 cell lines were examined by Western blot.
RESULTS: MTT assay showed that miR-200c could inhibit the proliferation of MDA-MB-231 and BT-549 cells. Wound-healing assay results showed that miR-200c could inhibit the migration of MDA-MB-231 and BT-549 cells. Western blot results showed that miR-200c up-regulated the expression of E-cadherin protein and down-regulated the expression of Vimentin protein.
CONCLUSIONS: The results showed that miR-200c can inhibit the proliferation of highly invasive human breast cancer cells and may inhibit cell migration by up-regulating the expression of E-cadherin protein and down-regulating the expression of Vimentin protein.

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