MiR-497 inhibits cell proliferation and invasion ability by targeting HMGA2 in pancreatic ductal adenocarcinoma

Z.-G. Zhou, C. Xu, Z. Dong, Y.-P. Wang, J.-Y. Duan, C.-Q. Yan

Department of General Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China. egwhfk@163.com

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• Oncology

OBJECTIVE: MicroRNAs have been implicated to play a crucial regulating role in human cancers. The study aims to explore the role and clinical significance of miR-497 in pancreatic ductal adenocarcinoma (PDAC).

PATIENTS AND METHODS: The relative expression of miR-497 in human PDAC tissue samples and adjacent normal tissues was measured using the quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Cell Counting Kit (CCK-8) assay, cell migration, and invasion assays were performed to detect cell proliferation and invasion ability. Downstream target gene was confirmed by using luciferase activity assays. QRT-PCR and Western blotting assays also were performed.

RESULTS: We found that miR-497 expression was significantly downregulated in PDAC tissues and cells. Lower miR-497 expression associated with lymph node metastasis and predicts a poor prognosis in PDAC patients. In in vitro assay, we demonstrated that miR-497 overexpression inhibited cell proliferation, migration, and invasion of PDAC. Furthermore, we demonstrated that HMGA2 was a direct target of miR-497 in PDAC cells. MiR-497 inhibited cell proliferation and invasion by regulating HMGA2 expression.

CONCLUSIONS: Our results indicated that miR-497 may serve as a predictor for PDAC and could be a novel target of PDAC treatment.

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