Long non-coding RNA PCAT-1 promotes cardiac fibroblast proliferation via upregulating TGF-β1

Q. Chen, C. Feng, Y. Liu, Q.-F. Li, F.-Y. Qiu, M.-H. Wang, Z.-D. Shen, G.-S. Fu

Department of Cardiology, Biomedical Research Center, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China. fugs@zju.edu.cn

---

• Cardiology

OBJECTIVE: Recently, the vital functions of long non-coding RNAs (lncRNAs) in many diseases have been explored. This study aims to identify the function of lncRNA PCAT-1 in the development of atrial fibrillation (AF).

PATIENTS AND METHODS: Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was performed to detect PCAT-1 expression in right atrial appendage (RAA) tissues of 51 AF patients and 35 patients with sinus rhythm (SR). Besides, cell proliferation assay was conducted in AC16 cells with PCAT-1 knockdown. Molecular mechanism of PCAT-1 in influencing the progression of AF was finally investigated.

RESULTS: PCAT-1 expression was higher in RAA tissues of AF patients than those of SR patients. Moreover, knockdown of PCAT-1 inhibited proliferation in AC16 cells. Transforming growth factor-β1 (TGF-β1) was a target of PCAT-1 and its expression in AF tissues positively correlated to PCAT-1 expression.

CONCLUSIONS: PCAT-1 could promote cell proliferation of AF via promoting TGF-β1, which may provide a new theory for AF development.

This article has been withdrawn. The Publisher apologizes for any inconvenience this may cause.

Free PDF Download