MiR-93-5p inhibited proliferation and metastasis of glioma cells by targeting MMP2

H. Wu, L. Liu, J.-M. Zhu

Department of Neurosurgery, The Second Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, China. dr.zhujunming@zju.edu.cn

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• Oncology

OBJECTIVE: To investigate the association between microRNA-93-5p (miR-93-5p) and glioma, and to explore the possible mechanisms.

PATIENTS AND METHODS: The expression level of miR-93-5p was detected in clinical tissue samples and cell lines. Online prediction websites were used to screen target of miR-93-5p, luciferase reporter assay and Western blot were performed to further confirm. The effects of the miR-93-5p on cell function were determined on U87-MG cells by in vitro experiments.

RESULTS: The low expression of miR-93-5p in glioma was confirmed by quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) assay. In Target gene prediction, matrix metalloproteinase-2 (MMP2) was identified as a direct target of miR-93-5p. The subsequent experiments showed that decreased expression of MMP2 resulting from the up-regulation of miR-93-5p obstructed the cell proliferation ability of U87-MG cells, and G0/G1 block occurred during the growth cycle. Further, the invasion and migration ability were also been affected.

CONCLUSIONS: Our research emphasized the suppressive function of miR-93-5p in glioma by targeting MMP2, thus providing some novel experimental basis for the treatment of glioma.

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