Role of miR-579-3p in the development of squamous cell lung carcinoma and the regulatory mechanisms

R.-R. Wu, Q. Zhong, H.-F. Liu, S.-B. Liu

Department of Oncology, Ganzhou People’s Hospital, Ganzhou, China. wrrsums@126.com

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• Oncology

OBJECTIVE: The aim of this study was to explore the role of microRNA-579-3p (miR-579-3p) in the development of squamous cell lung carcinoma (SCLC). Our findings might provide new insights into the treatment of SCLC.

PATIENTS AND METHODS: Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was performed to examine the expression level of miR-579-3p in 30 pairs of CRC tissues and para-cancerous tissues. The relation between miR-579-3p expression and clinical features of SCLC patients was analyzed. MiR-579-3p expression in SCLC cells was further verified by qRT-PCR as well. In addition, the effects of miR-579-3p on the migration and invasion of SK-MES-1 cells were examined through the transwell assay.

RESULTS: QRT-PCR results revealed for the first time that miR-579-3p was significantly down-regulated in SCLC tissues. This indicated that miR-579-3p was possibly involved in the development of SCLC. According to bioinformatics prediction websites and the luciferase activity assay, macrophage scavenger receptor 1 (MSR1) was predicted and verified as the target gene of miR-579-3p. Transfection of miR-579-3p mimics significantly reduced the protein expression level of MSR1 in cells, eventually inhibiting the proliferation, invasion, and migration of SCLC cells.

CONCLUSIONS: The miR-579-3p/MSR1 axis might be a novel regulatory pathway of apoptosis, which could be used as potential therapeutic sites in SCLC.

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