MiR-125a-5p inhibits EMT of ovarian cancer cells by regulating TAZ/EGFR signaling pathway

Y. Cao, T. Shen , C. Zhang, Q.-H. Zhang, Z.-Q. Zhang

Department of Obstetrics and Gynecology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. 1589766735@qq.com

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• Oncology

OBJECTIVE: To investigate the influences of micro ribonucleic acid (miR)-125a-5p on epithelial-mesenchymal transition (EMT) of ovarian cancer cells by regulating the transcriptional co-activator with PDZ-binding motif (TAZ)/epidermal growth factor receptor (EGFR) signaling pathway.

PATIENTS AND METHODS: The human ovarian cancer cells were cultured, and miR-125a-5p was repressed by inhibitor and overexpressed by miRNA mimics. The expression of EMT-related proteins was measured via Western blotting (WB). The action target of miR-125a-5p was determined through a dual-luciferase reporter gene assay. The changes in protein levels were detected via WB.

RESULTS: MiR-125a-5p was down-regulated remarkably in ovarian cancer tissues. The expression level of serum miR-125a-5p in patients with ovarian cancer was lower than that in control group. After inhibition on miR-125a-5p, the expression level of E-cadherin, an epithelial indicator, was decreased, while that of Vimentin, an interstitial indicator, was increased. MiR-125a-5p contained a complementary site in the 3’-untranslated region (UTR) of TAZ messenger RNA (mRNA). The expressions of TAZ mRNA and protein in cells were down-regulated markedly after the overexpression of miR-125a-5p. The expressions of EGFR, phosphorylated EGFR (p-EGFR) and p-Akt were up-regulated in the cells transfected with miR-125a-5p mimics and those transfected with miR-125a-5p mimics overexpressing TAZ.

CONCLUSIONS: MiR-125a-5p can inhibit the EMT of ovarian cancer cells by regulating the TAZ/EGFR signaling pathway.

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