LncRNA ITGB1 promotes the development of bladder cancer through regulating microRNA-10a expression

L. Dai, C.-M. Chai, T.-Y. Shen, Y. Tian, Z.-Q. Shang, Y.-J. Niu

Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin, China. urologyszq@sina.com

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• Oncology

OBJECTIVE: This study aims to investigate the expression level of lncRNA ITGB1 both in bladder cancer (BCa) tissue and cell lines, as well as to evaluate its function and potential mechanism in the progression of BCa.

PATIENTS AND METHODS: The expressions of lncRNA ITGB1 in 36 BCa tissues samples (and corresponding adjacent normal ones) and cell lines were detected by quantitative real-time polymerase chain reaction (qRT-PCR). After transfection of sh-ITGB1 in BCa cell lines, the effect of ITGB1 on the proliferation of BCa cells was examined by cell counting kit-8 (CCK-8) assay and colony formation assay. Subsequently, qRT-PCR was used to examine microRNA-10a expression in BCa tissues and cells after ITGB1 was silenced. At the same time, the correlation between ITGB1 and microRNA-10a expression was analyzed. Finally, cell recovery experiment was applied for the in-depth study of the interaction between ITGB1 and microRNA-10a and its underlying mechanism.

RESULTS: LncRNA ITGB1 was found upregulated in BCa tissues and cell lines. Knockdown of lncRNA ITGB1 remarkably inhibited cell proliferation. The expression levels of ITGB1 and microRNA-10a in BCa tissues were negatively correlated. ITGB1 downregulation was found to be able to enhance microRNA-10a expression, suggesting that microRNA-10a may be a potential target for ITGB1 in BCa. In addition, cell reverse experiment also verified that ITGB1 could regulate the expression of microRNA-10a, and their interaction affected the malignant progression of BCa.

CONCLUSIONS: LncRNA ITGB1 level is upregulated in BCa tissues and associated with the pathological stage of BCa, which could be used as a new predictor of BCa patients’ prognosis. In addition, ITGB1 might promote BCa cell proliferation via regulating microRNA-10a expression.

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