Long noncoding RNA LUCAT1 promotes cervical cancer cell proliferation and invasion by upregulating MTA1

A.-H. Wang, J.-M. Zhao, J. Du, Q.-X. Pang, M.-Q. Wang

Yan’an Key Laboratory of Cancer Prevention and Treatment, School of Medicine, Yan’an University, Yan’an, China. mingquan-wang@163.com

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• Oncology

OBJECTIVE: Recent researches have revealed the role of long noncoding RNAs (lncRNAs) in the development of tumors. In this study, lncRNA LUCAT1 was explored to identify how it affected the progression of cervical cancer.

PATIENTS AND METHODS: Quantitative Real-time polymerase chain reaction (qRT-PCR) was used to detect LUCAT1 expression in both cervical cancer cells and tissue samples. Moreover, the associations between LUCAT1 expression level and patients’ overall survival rate were explored, respectively. In addition, cell proliferation assay and transwell assay were conducted. Furthermore, the underlying mechanism was explored via performing qRT-PCR and Western blot assay.

RESULTS: By comparing with the expression level in corresponding ones, the LUCAT1 expression level in cervical cancer samples was significantly higher. Moreover, expression level of LUCAT1 was negatively correlated with patients’ overall survival time. In addition, after LUCAT1 was overexpressed, cell proliferation, cell invasion and migration capacities were promoted in vitro. In addition, the mRNA and protein expressions of MTA1 were upregulated after LUCAT1 was overexpressed. Furthermore, it was found that the expression level of MTA1 was positively related to LUCAT1 expression level in cervical cancer tissues.

CONCLUSIONS: We showed that LUCAT1 could enhance proliferation, invasion and migration of cervical cancer cells through upregulating MTA1, which might offer a potential therapeutic choice for patients with cervical cancer.

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