Verification of expressions of lncRNA FOXCUT in gastric adenocarcinoma patients and its effects on cell biological function based on TCGA database

D.-L. Zhao, G. Shen

Department of Gastrointestinal Surgery, Zhuji Affiliated Hospital of Wenzhou Medical University, Shaoxing, P.R. China. dsjv28@163.com

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• Oncology

OBJECTIVE: This study aimed to investigate expressions of lncRNA FOXCUT in gastric adenocarcinoma patients and its effects on the cell biological function.

PATIENTS AND METHODS: Expressions and survival of lncRNA FOXCUT in gastric adenocarcinoma patients (GA) in the Cancer Genome Atlas (TCGA) database were collected. Fifty patients with GA treated in our hospital (patient group) and another 50 contemporaneous normal people (normal group) were collected. Expressions of lncRNA FOXCUT in GES1, SNU-5, HGC-27, SGC-7901, and AGS cells were detected. Also, si-lncRNA FOXCUT and si-NC sequences were transfected to SGC-7901. Si-RNA and si-NC groups were constructed in AGS cells. QRT-PCR was used to detect expressions of lncRNA FOXCUT in samples. MTT, transwell, and flow cytometry were used to detect the proliferation, invasion, and apoptosis of transfected cells. Patients were followed up for 5 years to observe their survival.

RESULTS: Expressions of lncRNA FOXCUT in cancer tissues of GA patients in TCGA database were significantly increased (p<0.001). The survival rate of patients with low expressions of lncRNA FOXCUT was significantly increased (p=0.017, p=0.047). LncRNA FOXCUT is closely related to patients’ tumor diameter, lymph node metastasis, TNM staging, and differentiation degree (p<0.05). LncRNA FOXCUT has high clinical value in disease diagnosis. Multivariate Cox regression analysis found that tumor diameter, lymph node metastasis, and lncRNA FOXCUT were independent prognostic factors. Compared with GES1, expressions of lncRNA FOXCUT in GA cells increased significantly (p<0.05), the proliferation and invasion ability of si-RNA group decreased significantly (p<0.05) compared with si-NC group, and the apoptosis rate of si-RNA group was significantly lower than that of si-NC group (p<0.05).

CONCLUSIONS: We showed that the inhibition of the expressions of lncRNA FOXCUT can reduce the proliferation and invasion of GA cells and increase apoptosis, which can be used as a potential therapeutic target for GA.

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