MicroRNA-93-5p/IFNAR1 axis accelerates metastasis of endometrial carcinoma by activating the STAT3 pathway

J.-B. Xu

Department of Obstetrics and Gynecology, Zhenhai People’s Hospital, Ningbo, China. xiaoqingjb1217@126.com

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• Oncology

OBJECTIVE: The aim of this study was to elucidate whether microRNA-93-5p/IFNAR1 axis promoted proliferative and metastatic changes of endometrial carcinoma (EC) cells by regulating the STAT3 signaling pathway.

PATIENTS AND METHODS: The expression of microRNA-93-5p in EC tissues and cells was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Western blot was conducted to detect the protein expression of IFNAR1 in EC cells. Chi-square test was used to analyze the relationship between microRNA-93-5p expression and pathological characteristics of EC patients. The negative control, microRNA-93-5p mimics, microRNA-93-5p inhibitor or IFNAR1 vector were transfected into EC cells. Moreover, the proliferative and migratory potentials of EC cells were determined by cell counting kit-8 (CCK-8) and transwell assay, respectively.

RESULTS: MicroRNA-93-5p expression was significantly upregulated in EC tissues and cells. High expression of microRNA-93-5p indicated poor survival of EC patients. The microRNA-93-5p expression level was correlated with FIGO stage and lymph node metastasis of EC. Meanwhile, a negative correlation was found between microRNA-93-5p and IFNR1 in EC tissues. Furthermore, the overexpression of microRNA-93-5p remarkably increased the viability and migratory rate of EC cells, which were reversed by IFNR1 up-regulation.

CONCLUSIONS: MicroRNA-93-5p/IFNAR1 axis accelerates metastasis of EC through the STAT3 pathway.

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