LncRNA LUCAT1 promotes rowth, migration, and invasion of oral squamous cell carcinoma by upregulating PCNA

Y. Kong, Y. Feng, Y.-Y. Xiao, S.-C. Liu, X.-G. Li, Q.-L. Yang, W.-H. Chu, J.-G. Liu

Department of Pedodontics, Affiliated Stomatological Hospital of Jiamusi University in Heilongjiang Province, Jiamusi, China.lixiaoguangxiaoxia@163.com

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• Oncology

OBJECTIVE: Recent studies have revealed the important role of long noncoding RNAs (lncRNAs) in the progression of tumorigenesis. This study aims to identify how lncRNA LUCAT1 functions in the progression of OSCC (oral squamous cell carcinoma).

PATIENTS AND METHODS: Relative expression of lncRNA LUCAT1 in both OSCC cells and 50 paired tissue samples was detected by quantitative Real-time polymerase chain reaction (qRT-PCR). Moreover, biological function of LUCAT1 in OSCC was identified by performing transwell assay, wound healing assay and proliferation assay in vitro. The underlying mechanism was explored by qRT-PCR and Western blot.

RESULTS: LUCAT1 expression was remarkably downregulated in OSCC tissues when compared with that in adjacent normal samples. Moreover, proliferation, invasion and migration of OSCC cells were inhibited after knockdown of LUCAT1 in vitro. Knockdown of LUCAT1 downregulated PCNA in OSCC cells at mRNA and protein level in vitro. Besides, PCNA expression in OSCC tissues was positively correlated with the expression of LUCAT1.

CONCLUSIONS: Knockdown of LUCAT1 could inhibit migration, invasion and proliferation capacities of OSCC cells through downregulating PCNA, which may offer a new therapeutic intervention for OSCC patients.

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