Long noncoding RNA LUCAT1 promotes migration and invasion of prostate cancer cells by inhibiting KISS1 expression

C. Liu, L. Wang, Y.-W. Li, Y.-S. Cui, Y.-Q. Wang, S. Liu

Department of Urology, Qilu Hospital of Shandong University, Jinan, China. liushuangdoctor888@163.com

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• Oncology

OBJECTIVE: Recent researches have revealed the role of long noncoding RNAs (lncRNAs) in tumor development. In this study, the potential function of lncRNA LUCAT1 in the progression of prostate cancer was identified.
PATIENTS AND METHODS: Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was used to detect LUCAT1 expression in both prostate cancer cells and tissue samples. Moreover, the association between LUCAT1 expression level and overall survival of prostate cancer patients was analyzed. In addition, wound healing assay and transwell assay were conducted to evaluate the regulatory effect of LUCAT1 on prostate cancer cells. Furthermore, the underlying mechanism of LUCAT1 in regulating the development of prostate cancer was explored via qRT-PCR and Western blot.
RESULTS: LUCAT1 expression was much higher in prostate cancer samples than controls. Besides, LUCAT1 expression was correlated with the overall survival of prostate cancer patients. Moreover, LUCAT1 overexpression promoted in vitro migration and invasion of prostate cancer cells. In addition, the mRNA and protein expression of KISS1 were downregulated after LUCAT1 overexpression. Furthermore, it was found that the expression level of KISS1 was negatively related to the expression level of LUCAT1 in prostate cancer tissues.
CONCLUSIONS: LUCAT1 could enhance migration and invasion of prostate cancer cells by regulating KISS1, which might offer a potential therapeutic target for prostate cancer.

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