LINC01308 accelerates the malignant progression of ovarian cancer by binding to miRNA-506

Y.-Y. Zhang, P. Li, M.-Z. Zhu, Y. Guo, J. Yang

Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, China. 13507182023@163.com

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• Oncology

OBJECTIVE: To detect the expression pattern of LINC01308 in ovarian cancer (OC), and further clarify whether LINC01308 could promote the malignant progression of OC by mediating microRNA-506 (miRNA-506).
PATIENTS AND METHODS: Relative level of LINC01308 in 28 pairs of OC tissues and paracancerous tissues was determined by quantitative Real-time polymerase chain reaction (qRT-PCR). We analyzed the correlation between LINC01308 level and the prognosis of OC patients. Subsequently, LINC01308 level in OC cell lines was determined as well. By transfection of sh-LINC01308 in 3AO and CAOV3 cell lines, we evaluated the influence of LINC01308 on cellular behaviors of OC through cell counting kit-8 (CCK-8), transwell and wound healing assay. Target downstream of LINC01308 was verified by dual-luciferase reporter gene assay. Finally, the regulatory effect of LINC01308/miRNA-506 on OC cell behaviors was examined through a series of rescue experiments.
RESULTS: LINC01308 was highly expressed in OC tissues relative to controls. OC patients with high-level LINC01308 had higher rates of lymph node metastasis and distant metastasis, and lower survival rate compared with those with low level. By transfection of sh-LINC01308 in OC cells, the migratory and invasive abilities were markedly weakened. MiRNA-506 was found to be the target gene of LINC01308, and its level was negatively regulated by LINC01308 in OC tissues. Finally, we found that miRNA-506 knockdown reversed the regulatory effect of LINC01308 on the migratory and invasive abilities of OC cells.
CONCLUSIONS: LINC01308 is highly expressed in OC and correlated to metastasis and poor prognosis. LINC01308 enhances OC cells to migrate and invade by targeting miRNA-506.

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