Mechanism of decorin protein inhibiting invasion and metastasis of non-small cell lung cancer

Y. Gao, H.-Y. Ma, Q.-Y. Xu, Y. Li, S.-L. Zhang, Y.-Y. Qu, Y. Zhang, H. Yin

Department of Radiotherapy, Harbin Medical University Cancer Hospital, Harbin, P. R. China. mahongyu198405@163.com

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• Oncology

OBJECTIVE: To detect the protein level of Decorin in non-small cell lung cancer (NSCLC) patients, and to study the mechanism of Decorin inhibiting invasion and metastasis of non-small cell lung cancer from the perspective of in vitro cells, and provide some theoretical support for the treatment of non-small cell lung cancer.

MATERIALS AND METHODS: Immunohistochemical staining was used to detect the expression of Decorin protein in 332 cases of stage I-IIIA clinical NSCLC and 70 cases of adjacent tissues. Then, in vitro cell experiments (cell scratch assay and transwell assay) were used to further study the effects of Decorin on migration and invasion of human lung cancer cell line A549; the effect of transforming growth factor-β on the expression of Decorin and Ets-1 protein was verified by Western blotting. The binding sites of Ets-1 and Decorin promoter were analyzed by bioinformatics.

RESULTS: Exogenous Decorin inhibited invasion and metastasis of lung adenocarcinoma cells in vitro. Immunohistochemical staining showed that Decorin was lowly expressed in non-small cell lung cancer and Decorin had a certain effect on lung fibroblast activation. Western blotting results showed that TGF-β affects the expression of Decorin and Ets-1. Bioinformatics results showed that Ets-1 and Decorin gene DNA promoter regions have 18 binding sites.

CONCLUSIONS: Decorin inhibits invasion and metastasis of non-small cell lung cancer through the TGF-β signaling pathway.

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