MiR-182-5p inhibited proliferation and metastasis of colorectal cancer by targeting MTDH

Y. Jin, Z.-L. Zhang, Y. Huang, K.-N. Zhang, B. Xiong

Department of Gastrointestinal Surgery, Zhongnan Hospital of Wuhan University, Wuhan, China. binxiong1961@163.com

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• Oncology

OBJECTIVE: To investigate the role and molecular mechanism of miR-182-5p in the development of colorectal cancer (CRC), thereby providing a theoretical basis for new CRC therapeutic targets.

PATIENTS AND METHODS: The expression of miR-182-5p in CRC clinical cases and biological cell lines was detected. On-line target gene prediction and Luciferase reporter gene assay were performed to screen and verify the target of miR-182-5p, respectively. The role of miR-182-5p in CRC cell function was further analyzed.

RESULTS: MiR-182-5p expression was significantly decreased in both CRC tissues and cell lines. Metadherin (MTDH) was screened and verified as a functional target of miR-182-5p. The cell proliferation, invasion and migration ability of CRC cells were significantly inhibited after the up-regulation of miR-182-5p. However, MTDH limited the anti-cancer effects of miR-182-5p in CRC cells.

CONCLUSIONS: Our research demonstrated the inhibitory function of miR-182-5p in CRC. Therefore, the miR-182-5p/MTDH axis was expected to be one of the targets of CRC targeted therapy.

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