MiR-374b reduces cell proliferation and cell invasion of cervical cancer through regulating FOXM1

N. Xia, W.-F. Tan, Q.-Z. Peng, H.-N. Cai

Department of Gynaecology, Maternal and Child Health Hospital of HuBei Province, Wuhan, China. 493946385@qq.com

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• Oncology

OBJECTIVE: Deregulation of microRNAs (miRNAs) has been identified as critical event in tumor initiation and progression. We aimed to explore the role of miR-374b in cervical cancer progression.

PATIENTS AND METHODS: MiR-374b expression was detected using qRT-PCR in cervical cancer tissues compared with normal counterparts. Cell proliferation and invasion ability were detected using Cell Counting Kit-8 (CCK8) cell proliferation and transwell invasion assay. Dual luciferase reporter assay, qRT-PCR, and Western blot analysis were used to demonstrate that FOXM1 was a target of miR-374b.

RESULTS: We demonstrated that downregulation of miR-374b was frequently examined in cervical cancer tissues compared with normal counterparts. Furthermore, we showed the lower miR-374b expression associated with lymph node metastasis and advanced FIGO stage in patients with cervical cancer. Furthermore, ectopic expression of miR-374b could significantly decrease cell proliferation and invasion ability. However, inhibition of miR-374b had opposite effects. Dual luciferase reporter assay, qRT-PCR, and Western blot analysis revealed that miR-374b overexpression suppressed cell proliferation and invasion ability via affecting FOXM1 expression.

CONCLUSIONS: These results indicated that miR-374b acted as tumor suppressor and may serve as a potential target for cervical cancer treatment.

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