Effects of normal lymphatic fluid on rats with sepsis complicated with lung injury

Y.-M. Li, Y. Jiang, S.-D. Feng, Z.-H. Lin

Department of Emergency, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China. w75791799151113@sina.com

---

• Pharmacology

OBJECTIVE: Infection can be caused by severe burnt, trauma and hypoxia, further causing systemic inflammatory syndrome or sepsis. The sepsis occurs in about 2% of all hospitalizations and ranges from 6% to 30% in intensive care unit (ICU) in developed countries This study aimed to investigate the effects of normal lymph fluid on sepsis complicated with pulmonary injury.

MATERIALS AND METHODS: Wistar rats were prepared for sepsis complicated with acute pulmonary model via cecal ligation and puncture, and received normal lymph fluid injection 60 min later. Artery blood-gas index, wet/dry weight (W/D) ratio of lung, Myeloperoxidase (MPO) and superoxide dismutase (SOD) activity of lung tissues were measured, along with protein content and cell count in bronchoalveolar lavage fluid (BALF). Real-time PCR (RT-PCR) and Western blot were employed to measure expression of nuclear factor κB (NF-κB) in lung tissues, whilst enzyme linked immunosorbent assay (ELISA) was used to analyze serum expression of tumor necrosis factor α (TNF-α) and interleukin 2 (IL-2).

RESULTS: Model group had significantly depressed PaO2 and pH value, higher W/D ratio, and MPO activity, lower SOD activity, higher protein and cell count of BALF, and up-regulation of TNF-α, IL-2 and NF-κB (p < 0.05 compared to sham group). Infusion of lymph fluid effectively improved blood-gas function, decreased W/D ratio, MPO activity, elevated SOD activity, and lowered TNF-α, IL-2 and NF-κB expression (p < 0.05 compared to model group).

CONCLUSIONS: Normal lymph fluid can inhibit NF-κB expression, suppress inflammation, and improve blood-gas exchange in lung tissues. Therefore, the normal lymph fluid could effectively relieve the sepsis complicated with pulmonary injury.

 

Free PDF Download