HIF-1α restricts proliferation and apoptosis of Tca8113 cells through up regulation of Hippo signaling pathway under hypoxic conditions

J.-Y. Chen, Y.-G. Zhang, J.-D. Du

Department of Oral General, Lanzhou Del Dental Hospital of Gansu Province, Lanzhou, Gansu, China. pingyi228224@163.com

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• Oncology

OBJECTIVE: The hypoxia-inducible factor-1α (HIF-1α) is a key factor for tumor cells adaptation to hypoxia. Studies have shown that under hypoxic conditions, HIF-1α expression was significantly increased in human tongue squamous cell carcinoma cells (Tca8113). This research aims to determine the exact mechanism of HIF-1α on the proliferation and apoptosis of Tca8113 cells.

MATERIALS AND METHODS: Tca8113 cells were cultured under normoxia and hypoxia. Real Time-PCR and Western blot were used to measure the expression levels of HIF-1α and TAZ. Under hypoxic condition, HIF-1α siRNA was transfected into Tca8113 cells. CCK8 was used to measure the proliferation of Tca8113 cells. Flow cytometry was used to detect apoptosis of Tca8113 cells.

RESULTS: Under hypoxic condition, the expression levels of HIF-1α and TAZ at both mRNA and protein levels were significantly increased (p <0.05). The downregulation of HIF-1α by siRNA significantly inhibited Tca8113 cells proliferation, increased their apoptosis, and reduced the expression level of TAZ.

CONCLUSIONS: Under hypoxic conditions, HIF-1α inhibits the proliferation and apoptosis of Tca8113 cells via the elevation of the Hippo signaling pathway.

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