Protective effect of cyclosporine on inflammatory injury of renal tubular epithelial cells

Y.-Q. Zhang, Y. Chen, Y.-M. Ding, T.-H. Yu

Department of Nephrology, The Affiliated Jiangyin Hospital of Southeast University Medical College, Wuxi, China. 13626238242@163.com

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• Pharmacology

OBJECTIVE: This study aims to explore the protective effect of cyclosporine on inflammation-induced renal tubular epithelial cells and its potential mechanism.

MATERIALS AND METHODS: Human kidney-2 (HK-2) cells were induced by transforming growth factor-β (TGF-β) for constructing an inflammatory injury model. Cells were then treated with different concentrations of cyclosporine for further investigating the biological functions. Cell viability was detected via cell counting kit-8 assay (CCK-8). The cytotoxicity was detected via lactate dehydrogenase (LDH) release assay. Expression levels of cell damage factors and mammalian target of rapamycin (mTOR) pathway-related genes were detected via polymerase chain reaction (PCR), immunofluorescence and Western blotting, respectively.

RESULTS: TGF-β inhibited the viability of HK-2 cells, increased expressions of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and apoptosis-related genes. Cyclosporine treatment greatly reversed the cell damage on HK-2 cells induced by TGF-β. Expression levels of mTOR pathway-related genes were downregulated after cyclosporine treatment.

CONCLUSIONS: Cyclosporine protects HK-2 cells from inflammatory injury via regulating mTOR pathway.

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