MiR-363-3p modulates cell growth and invasion in glioma by directly targeting pyruvate dehydrogenase B

D.-X. Xu, J.-J. Guo, G.-Y. Zhu, H.-J. Wu, Q.-S. Zhang, T. Cui

Department of Neurosurgery, The First Affiliated Hospital of Henan University of Science and Technology, Henan, China. abuzo815@sina.com

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• Oncology

OBJECTIVE: This study is designed to investigate the role of miR-363-3p in the cancer development of glioma.

PATIENTS AND METHODS: The expression of miR-363-3p in glioma and adjacent noncancerous tissue was measured using quantitative RT-PCR. The expression of a target gene of miR-363-3p, pyruvate dehydrogenase B (PDHB), was determined by Western blot. The level of miR-363-3p was increased or decreased by transfected with miR-363-3p mimic or miR-363-3p inhibitor, respectively. The impact of miR-363-3p on cell growth, apoptosis and invasion was determined by CCK-8 (Cell Counting Kit) assay, flow cytometry, and transwell assay. The role of PDHB in mediating the oncogenic activities was demonstrated by co-transfected PDHB vector and miR-363-3p mimic.

RESULTS: Our results have shown that miR-3663-3p level was significantly higher in glioma tissue. Furthermore, miR-363-3p functions as onco-miRNA, promotes cell proliferation, protects against apoptosis, and enhances invasion by directly targeting PDHB.

CONCLUSIONS: MiR-363-3p is an onco-miRNA, which can be considered as a potential therapeutic target in glioma.

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