MiR-129 is involved in the occurrence of uterine fibroid through inhibiting TET1

J.-L. Lu, L. Zhao, S.-C. Han, J.-L. Bi, H.-X. Liu, C. Yue, L. Lin

Department of Gynecology and Obstetrics, The Second Hospital of Dalian Medical University, Dalian, China. bigcatlinlin@163.com

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• Gynecology

OBJECTIVE: To detect the expressions of micro ribonucleic acid (miR)-129 and its target gene in uterine fibroid tissues and to investigate the role of miR-129 in the occurrence of uterine fibroid.

PATIENTS AND METHODS: The expressions of miR-129 and its target gene ten-eleven translocation 1 (TET1) were detected via quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Dual-luciferase reporter gene and Western blotting were used to verify the regulatory relation between miR-129 and target gene. The effects of miR-129 on the proliferation, apoptosis, cycle and extracellular matrix (ECM) of uterine fibroid cells were investigated via transfection with miR-129 mimics and TET1 small-interfering RNA (siRNA).

RESULTS: MiR-129 was lowly expressed in uterine fibroid. The expression of miR-129 was regulated by sex hormones. The highly expressed miR-129 promoted apoptosis and inhibited proliferation through reducing the low expression of TET1. At the same time, miR-129 affected the accumulation of ECM.

CONCLUSIONS: The expression of miR-129 in uterine fibroid is lower, and the proliferation capacity of tumor cells is enhanced, thus promoting the occurrence and development of uterine fibroid.

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