Mir-98 reduces the expression of HMGA2 and promotes osteogenic differentiation of mesenchymal stem cells

X.-L. Gao, M.-G. Cao, G.-G. Ai, Y.-B. Hu

Department of Spine Surgery, Sixth Affiliated Hospital of Xinjiang Medical University, Ürümqi, China. hyb4499@126.com

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• Stem Cells

OBJECTIVE: To study effects of microRNA-98 (miR-98) on osteogenic differentiation of mesenchymal stem cells (MSCs).

PATIENTS AND METHODS: We predicted target gene of miR-98 with software test, and detected expression changes of miR-98, as well as its target gene HMGA2, in the process of osteogenic differentiation of mesenchymal stem cells. After transfection of miR-98 mimic and HMGA2 siRNA, we induced osteogenic differentiation and detected expression changes of osteogenic differentiation markers (RUNX2, ALP, OCN, and BSP).

RESULTS: MiR-98 combined directly with target gene HMGA2 and inhibited its expression. During the process of osteogenic differentiation, expression of miR-98 was up-regulated, while HMGA2 expression was down-regulated. In addition, the expression of osteogenesis maker genes increased in cells being transfected with miR-98 mimics and HMGA2 siRNA.

CONCLUSIONS: MiR-98 can promote osteogenic differentiation of mesenchymal stem cells by targeting gene HMGA2.

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