Inhibition of thyroid carcinoma cells with YAP1 protein interference and its mechanism

C.-Y. Huang, Z. Han, X. Li, H.-H. Xie, S.-S. Zhu

Department of Breast and Thyroid Surgery, Hexian Memorial Hospital of Panyu District, Guangzhou, China. allenhz520@163.com

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• Oncology

OBJECTIVE: To investigate the effects and mechanism of yes-associated protein 1 (YAP1) on thyroid carcinoma cells.

MATERIALS AND METHODS: Quantitative Real-time PCR (qRT-PCR) and Western blot assay were used to detect the expression of YAP1 in normal thyroid cells (HT-ori3) and four types of thyroid carcinoma cells: FTC-133, IHH-4, TPC-1 and NPA. The cell lines with the highest expression of YAP1 were selected as the experimental materials. qRT-PCR and Western blot assay were used to detect the interference effect of si-YAP1. The cell proliferation and the effect on the PI3K-Akt signal pathway were examined by MTT and Western blot.

RESULTS: The expression of YAP1 significantly increased in the thyroid carcinoma cell line compared with normal thyroid cells, among which the expression of YAP1 in TPC-1 was the highest. Quantitative PCR and Western blot results showed significant interference effects. The MTT assay indicated that YAP1 interference suppressed the proliferation of cells and the expression of p-Akt.

CONCLUSIONS: The interference of YAP1 can inhibit the growth of thyroid cancer cells, and its mechanism may be associated with the PI3K-Akt signaling pathway.

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