Effect of lncRNA LET on proliferation and invasion of osteosarcoma cells

G. Kong, X.-J. Qi, J.-F. Wang

Department of Joint Orthopedics, Yantaishan Hospital, Yantai, Shandong, China. wjfdoc@126.com

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• Oncology

OBJECTIVE: To investigate the expression of long non-coding RNA (LncRNA) LET in osteosarcoma and its effect on the proliferation, apoptosis, migration and invasion of osteosarcoma cells.

PATIENTS AND METHODS: The expression of lncRNA LET was detected in osteosarcoma tissues and cell lines (MG63 and hFOB1.19). MG63 cells stably overexpressing lncRNA LET were constructed by lentiviral. The effects of lncRNA LET overexpression on the proliferation, apoptosis, migration and invasion of osteosarcoma cells were detected by cell counting kit-8 (CCK-8), flow cytometry and transwell chamber assay.

RESULTS: The expression of lncRNA LET in osteosarcoma tissues and MG63 cells was significantly down-regulated. Overexpression of lncRNA LET significantly inhibited the proliferation, migration, invasion, and induced apoptosis of MG63 cells.

CONCLUSIONS: LncRNA LET was participated in the development of osteosarcoma, and may be used as a potential molecular target for the treatment of osteosarcoma.

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