Effect of TAGLN2 in the regulation of meningioma tumorigenesis and development

J. Pei, P. Li, Z.-Y. Zhang, H.-L. Zhang, Y.-H. Gao, D.-Y. Wang, Y. Zheng, X. Xu, J.-Z. Cui

Department of Interventional Neuroradiology, Tangshan Gongren Hospital, Tangshan, China. ben_pjpj@hotmail.com

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• Oncology

OBJECTIVE: To explore the role of transgelin-2 TAGLN2 in the development and progression of meningioma and the potential regulatory.

MATERIALS AND METHODS: TAGLN2 knockdown expression and overexpression in vitro models were constructed using lentivirus in meningioma cell line CH157; their corresponding transfection efficiencies were verified by qRT-PCR and Western Blot. Actions of TAGLN2 on the proliferation of meningioma cells were explored by CCK8 and colony formation assays. The effect of TAGLN2 on invasion of meningioma cells was analyzed by transwell cell invasion assay. Biological function of TAGLN2 on apoptosis of meningioma cells was determined by flow cytometry. Finally, Western Blot was used to investigate the detailed mechanism of TAGLN2 on regulating the biological functions of meningioma cells.

RESULTS: After down-regulating the expression of TAGLN2, there were significantly decreased capacities of cells proliferation and colony formation of meningioma cells, meanwhile, cell invasion was significantly decreased but the apoptosis rate was increased. On the contrary, up-regulation of TAGLN2 expression, the proliferation, colony formation ability were significantly increased as well as the invasion capacity, whilst apoptosis rate was decreased. Western Blot showed that expressions of p-PI3K and p-AKT were inhibited after knockdown of TAGLN2, which were significantly increased after TAGLN2 was overexpressed.

CONCLUSIONS: TAGLN2 can affect the proliferation, invasion and apoptosis of meningioma cells and may participate in the development of meningioma through regulating the PI3K/AKT signaling pathway.

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