Silencing of hsa_circ_0007534 suppresses proliferation and induces apoptosis in colorectal cancer cells

R. Zhang, J. Xu, J. Zhao, X. Wang

Department of Ophthalmology, the Fourth Affiliated Hospital, China Medical University, China. wxinling@126.com

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• Oncology

OBJECTIVE: Although some circRNAs have been found to regulate the progression of colorectal cancer (CRC). However, their functions have not been completely clarified. In the present study, a novel circRNA hsa_circ_0007534 was investigated in CRC tumor tissues and cell lines.

PATIENTS AND METHODS: The expression profile of circRNAs in CRC tumor tissues was performed by human circRNA microarray. The CCK-8 and apoptosis assay were measured in CRC cell lines after transfected with si-circRNA and si-NC.

RESULTS: We analyzed a cohort of 33 patients with CRC and demonstrated that the expression of hsa_circ_0007534 was significantly up-regulated in CRC tumor tissues as compared to adjacent non-tumorous tissues. Moreover, hsa_circ_0007534 expression was correlated with tumor stage and lymph node metastasis. Furthermore, the silence of hsa_circ_0007534 by siRNA significantly inhibited proliferation and induced apoptosis of CRC cells.

CONCLUSIONS: Hsa_circ_0007534 plays a crucial role in the initiation and progression of CRC and may be a potential therapeutic target of CRC.

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