Formyl peptide receptor 2 mediated chemotherapeutics drug resistance in colon cancer cells

L.-D. Su, J.-M. Peng, Y.-B. Ge

Department of Medical Yangzhou Polytechnic College, Yangzhou, China. ybge@njmu.edu.cn

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• Oncology

OBJECTIVE: To determine the expression of formyl peptide receptor 2 (FPRL2) and its drug resistance role in cancer colon cells, and its underlying mechanisms.

PATIENTS AND METHODS: The expression of FPRL2 and its legend (F2L) in colon cancer tissues or cancer cells was determined by immunohistochemistry assay and Real-time polymerase chain reaction (PCR), respectively. Chemosensitivity of 5-Fu and MMC in colon cancer cells were tested by cell counting kit-8 (CCK-8) method. Expression of p-ERK was determined by Western blot assay.

RESULTS: The expression of FPRL2 and its legend was significantly higher in resistant colon cancer tissues than those in non-resistant colon cancer tissues. The FPRL2 positive cells were two-thirds in tested cell lines. All of cells were F2L positive. The IC50 (inhibitory concentration 50) by 5-Fu and MMC was significantly higher in FPRL2 positive cells than those negative cells. The expression of p-AKT was markedly increased in FPRL2 positive cells. Pretreatment with AKT inhibitor enhanced the drug-sensitivity of these cells to 5-Fu and MMC.

CONCLUSIONS: The FPRL2 played a significant role in colon cancer drug resistance and this effect was through AKT pathway.

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