Down-regulation of survivin enhances paclitaxel-induced Hela cell apoptosis

F. Gu, L. Li, Q.-F. Yuan, C. Li, Z.-H. Li

Department of Obstetrics, Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China. leiliqwe@sina.com

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• Pharmacology

OBJECTIVE: Paclitaxel is one of the common anticancer drugs in the treatment of cervical cancer, while the mechanism of restraining and killing cancer cells is still unclear. This study aimed to investigate the molecular mechanism of paclitaxel in regulating proliferation and apoptosis of cervical cancer Hela cells.

MATERIALS AND METHODS: Paclitaxel at 2 μmol/L was used to treat Hela cells for 48 h. MTT assay and flow cytometry were applied to test Hela cells proliferation and apoptosis respectively. Western blot was adopted to determine the expression of survivin. SiRNA was performed to suppress survivin protein expression in Hela cells.

RESULTS: Paclitaxel restrained Hela cells growth and induced apoptosis. Also, paclitaxel treatment significantly reduced survivin protein expression in Hela cells. Moreover, survivin siRNA transfection further promoted Hela cells apoptosis after intervention by 2 μmol/L paclitaxel.

CONCLUSIONS: Down-regulation of survivin promoted paclitaxel-induced apoptosis of cervical cancer Hela cells.

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